Gene Expression in the Rat Brain During Prostaglandin D2 and Adenosinergically-Induced Sleep

Abstract

Previous studies have supported the hypothesis that macromolecular synthesis occurs in the brain during sleep as a response to prior waking activities and that prostaglandin D₂ (PGD₂) is an endogenous sleep substance whose effects are dependent on adenosine A_2a receptor-mediated signaling. We compared gene expression in the cerebral cortex, basal forebrain, and hypothalamus during PGD₂-induced and adenosinergically-induced sleep to results from our previously published study of recovery sleep (RS) after sleep deprivation (SD). Immediate early gene expression in the cortex during sleep induced by PGD₂– or by the selective adenosine A_2a agonist CGS21680 showed limited similarity to that observed during RS while, in the basal forebrain and hypothalamus, widespread activation of immediate early genes not seen during RS occurred. In all three brain regions, PGD₂ and CGS21680 reduced the expression of arc, a transcript whose expression is elevated during SD. Using GeneChips^®, the majority of genes induced by either PGD₂ or CGS21680 were induced by both, suggesting activation of the same pathways. However, gene expression induced in the brain after PGD₂ or CGS21680 treatment was distinct from that described during RS after SD and apparently involves glial cell gene activation and signaling pathways in neural-immune interactions.